Figure 3

IL-1β response to acute stressor exposure in adipocyte and stroma-vascular fractions of subcutaneous WAT. Effect of acute stressor exposure on the IL-1β protein content of stromal-vascular and adipocyte fractions of subcutaneous (inguinal) WAT in healthy, non-obese rats. 21 week old male F344 rats were exposed to 50 inescapable 1.5 mA tail shocks (stress, n = 3) or remained in their home cages (no stress, n = 3). Rats were sacrificed immediately following stressor exposure. Approximately 0.3 g of subcutaneous WAT was harvested and digested via standard WAT collagenase digestion [139]. The stroma-vascular and adipocyte fractions were then processed via homogenization in a RIPA lysis buffer. IL-1β protein analysis (pg IL-1β per mg total protein) was done via ELISA (R&D Systems Minneapolis, MN). Effect of fraction on IL-1β concentration F(1,8) = 15.751, ***P < 0.0001. Effect of stress on IL-1β content: F(1,8) = 7.031, *P < 0.05. Fraction by stress interaction: F(3,6) = 15.697, ##P < 0.01. Values represent group means + standard error of measurement. All experiment protocols were approved by the Animal Care and Use Committee of the University of Colorado at Boulder.