Figure 1

Time dependent effects of feeding dietary oils on leukocyte and platelet adhesion and rolling in jejunum venules of WT C57/BL6J mice. The extent of leukocyte and platelet trafficking in post-capillary venules in the jejunum wall of WT C57/BL6J mice was determined at various time points (1.0 h, 1.5 h, 2.0 h and 2.5 h) following gavage with olive oil or corn oil by intravital microscopy. [A] Leukocyte and platelet adherence to post-capillary venules was significantly increased 2 h after olive oil administration (*P < 0.05 vs. Saline control). Platelet adherence significantly declined by 2.5 h post following treatment (^#P < 0.05 vs. Olive 2.0 h group) and leukocyte adherence began to decline at 2.5 h but it was still higher than saline control (*P < 0.05 vs. Saline control) [B] Leukocyte and platelet rolling in post-capillary venules was also significantly increased 2.0 h after gavage with olive oil (*P < 0.05 vs. Saline control) and then significantly declined after 2.5 h. (^#P < 0.05 vs. Olive 2.0 h group) [C] Leukocyte and platelet adherence to post-capillary venules was significantly increased at 1.5 h, 2.0 h and 1.5 h was the peak time point for corn oil (*P < 0.05 vs. Saline control). They began to decline from 2.0 h post following gavage and platelet adherence significantly declined by 2.5 h post following treatment (^#P < 0.05 vs. Corn oil 1.5 h group) [D] Leukocyte and platelet rolling in post-capillary venules was also significantly increased at 1.5 h and 2.0 h (*P < 0.05 vs. Saline control) and then significantly declined after 2.5 h. (^#P < 0.05 vs. Corn 1.5 h group).