Figure 3

TLR4 deletion diminished leukocyte and platelet adherence and rolling induced by feeding dietary oils in jejunum venules of TLR4^-/-mice. B6.B₁₀ ScN-Tlr4^laps-del/JthJ (TLR4^-/-) mice were used as a TLR4-knockout strain, with wild type mice of this strain serving as controls. Intravital microscopy analysis of the jejunum venules was studied in these mice at their ‘peak’ time point respectively (2.0 h after olive oil gavage or 1.5 h after corn oil gavage). TLR4 deletion significantly diminished leukocyte and platelet adherence to post-capillary venules 2 h after gavage with olive oil (*P < 0.05 vs. WT + Saline control. ^#P < 0.05 vs. WT + Olive 2.0 h group) [A] or 1.5 h after gavage with corn oil (*P < 0.05 vs. WT + Saline control. ^#P < 0.05 vs. WT + Corn 1.5 h group) [B]. TLR4 deletion also significantly reduced leukocyte and platelet rolling in post-capillary venules induced by gavage with olive oil for 2 h (*P < 0.05 vs. WT + Saline control. ^#P < 0.05 vs. WT + Olive 2.0 h group) [C] or with corn oil for 1.5 h (*P < 0.05 vs. WT + Saline control. ^#P < 0.05 vs. WT + Corn 1.5 h group) [D].