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Figure 4 | BMC Physiology

Figure 4

From: The endotoxin/toll-like receptor-4 axis mediates gut microvascular dysfunction associated with post-prandial lipidemia

Figure 4

Effect of feeding dietary oils on TLR4 protein expression during postprandial lipemia. Samples of jejunum were collected following 2.0 h olive oil administration in WT + Saline, WT + Olive 2.0 h, WT + Olive 2.0 h + AB (pretreated with antibiotics for 7d) and TLR4-/- + Olive 2.0 h mice. TLR4 protein level was examined using standard western blotting techniques. TLR4 protein expression was significantly increased in mice gavaged with olive oil at 2 h (**P < 0.01 vs. WT + Saline control); this effect was significantly reduced in mice pretreated with antibiotics and in TLR4 knockout (TLR4-/-) mice (#P < 0.05 vs. WT + Olive 2.0 h group) [A]. Samples of jejunum were collected following 1.5 h corn oil administration in WT + Saline, WT + Corn 1.5 h, WT + Corn 1.5 h + AB (pretreated with antibiotics for 7d) and TLR4-/- + Corn 1.5 h mice. TLR4 protein level was examined using standard western blotting techniques. TLR4 protein expression was significantly increased in mice gavaged with corn oil at 1.5 h (**P < 0.01 vs. WT + Saline control); this effect was significantly reduced in mice pretreated with antibiotics and in TLR4 knockout (TLR4-/-) mice (#P < 0.05 vs. WT + Corn 1.5 h group) [B].

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