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Figure 5 | BMC Physiology

Figure 5

From: The endotoxin/toll-like receptor-4 axis mediates gut microvascular dysfunction associated with post-prandial lipidemia

Figure 5

Effect of feeding dietary oils on NF- k B p65 protein expression and phosphorylation during postprandial lipemia. Samples of jejunum were collected following 2.0 h olive oil administration in WT + Saline, WT + Olive 2.0 h, WT + Olive 2.0 h + AB (pretreated with antibiotics for 7d) and TLR4-/- + Olive 2.0 h mice. NF-k B p65 protein expression and phosphorylation was examined using standard western blotting techniques. pNF-κB p65 expression was significantly increased in mice gavaged with olive oil at 2 h (**P < 0.01 vs. WT + Saline control); this phosphorylation was significantly decreased in mice which had been pre-treated with antibiotics and in TLR4 knockout (TLR4-/-) mice (#P < 0.05 vs. WT + Olive 2.0 h group) [A]. Samples of jejunum were collected following 1.5 h corn oil administration in WT + Saline, WT + Corn1.5 h, WT + Corn 1.5 h + AB (pretreated with antibiotics for 7d) and TLR4-/- + Corn 1.5 h mice. NF-k B p65 protein expression and phosphorylation was examined using standard western blotting techniques. NF-k B p65 expression was significantly increased in mice gavaged with corn oil at 1.5 h (**P < 0.01 vs. WT + Saline control); this effect was significantly reduced in mice pretreated with antibiotics and in TLR4 knockout (TLR4-/-) mice (#P < 0.05 vs. WT + Corn 1.5 h group) [B]. However, total NF-k B p65 protein expression was not significantly different between these groups.

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