Figure 6

Arteriolar vasodilatation responses to Ach (10^-5M) and Pap in different oil groups. [A] The ability of arterioles to dilate in response to acetylcholine (Ach) and Pap in WT + Saline, WT + Olive 2.0 h, WT + AB + Olive 2.0 h and TLR4^-/- + Olive 2.0 h mice was measured by intravital microscopy. Arterioles of mice gavaged with olive oil for 2 h exhibited significantly impaired vasodilatation responses to Ach (*P < 0.05 vs. WT + Saline control). This phenomenon was however blunted in mice pre-treated with antibiotics and in TLR4 mutant mice but vasodilatory responses to papaverine, an endothelial-independent vasodilator, was not significantly different between these groups. (^#P < 0.05 vs. WT + Olive 2.0 h group). [B] The ability of arterioles to dilate in response to acetylcholine (Ach) and Pap in WT + Saline, WT + Corn 1.5 h, WT + AB + Corn 1.5 h and TLR4^-/- + Corn 1.5 h mice was measured by intravital microscopy. Arterioles of mice gavaged with corn oil for 1.5 h exhibited significantly impaired vasodilatation responses to Ach (*P < 0.05 vs. WT + Saline control). This phenomenon was however blunted in mice pre-treated with antibiotics and in TLR4 mutant mice but vasodilatory responses to papaverine, an endothelial-independent vasodilator were not significantly different between these groups. (^#P < 0.05 vs. WT + Corn 1.5 h group).