Ct, average |
---|
 Tissue | 18S | KCC1 | KCC2 | KCC3a | KCC3b | KCC4 |
rSCCD | Â | 26.4 | BT | 25.0 | 27.3 | 26.7 |
rat kidney | 14.2 | 25.9 | -- | 23.3 | 24.5 | 26.3 |
rat brain | -- | -- | 22.6 | -- | -- | -- |
blank | 35.0 | 30.2 | BT | BT | 32.3 | 35.8 |
- BT, Below Threshold fluorescence at end of 38 thermocycles. Ct values are based on duplicate runs of 4 samples SCCD and duplicates of control tissue total RNA. The difference between Ct values of duplicates varied by 0.3 ± 0.04 (n=26) with a range from 0.0 to 0.8.