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Table 1 RT-PCR demonstration of the presence of KCC1, KCC3a, KCC3b, KCC4 and absence of KCC2 in rat SCCD primary cultures

From: cAMP-stimulated Cl- secretion is increased by glucocorticoids and inhibited by bumetanide in semicircular canal duct epithelium

Ct, average

 Tissue

18S

KCC1

KCC2

KCC3a

KCC3b

KCC4

rSCCD

 

26.4

BT

25.0

27.3

26.7

rat kidney

14.2

25.9

--

23.3

24.5

26.3

rat brain

--

--

22.6

--

--

--

blank

35.0

30.2

BT

BT

32.3

35.8

  1. BT, Below Threshold fluorescence at end of 38 thermocycles. Ct values are based on duplicate runs of 4 samples SCCD and duplicates of control tissue total RNA. The difference between Ct values of duplicates varied by 0.3 ± 0.04 (n=26) with a range from 0.0 to 0.8.