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Table 2 RT-PCR demonstration of the presence of WNK1 and absence of WNK4 in rat SCCD primary cultures

From: cAMP-stimulated Cl- secretion is increased by glucocorticoids and inhibited by bumetanide in semicircular canal duct epithelium

Tissue

18S

WNK1

WNK4

rSCCD

13.4

24.7

31.6

rat kidney

14.7

25.0

26.0

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34.4

 BT

35.2

  1. Ct, average. BT, Below Threshold fluorescence at end of 38 thermocycles. Ct values are based on duplicate runs of 4 samples SCCD and duplicates of rat kidney as a control tissue. The difference between Ct values of duplicates varied by 0.6 ± 0.09 (n=36) with a range from 0.0 to 2.1.
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BMC Physiology

ISSN: 1472-6793

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