Table 3 Gene-specific primers
Gene | Primers 5′—3′ | GenBank # (species) | Amplicon size (bp) | Source |
|---|---|---|---|---|
18S | S: GAG GTT CGA AGA CGA TCA GA | BK000964 (many) | 316 | [65] |
AS: TCG CTC CAC CAA CTA AGA AC | ||||
KCC1 | S: GTT CGC CTC ACT CTT CCT GGC | U55815 (rat) | 419 | [66] |
AS: TGG GCC ACC ACA TAC AGG GA | ||||
KCC2 | S: CAT CAC AGA TGA ATC TCG GG | U55816 (rat) | 213 | [66] |
AS: TTC TCT GGG TCT GTC TCC C | ||||
KCC3a | S: CCT CGC CTC CTC ACC TTT GC | AF211854 (mouse) | 284 | This study |
AS: TCA CTC TGA CGC CAG CCA TTG | ||||
KCC3b | S: AGT AAA AGC CCG GAT TCA GG | AF211855 (mouse) | 330 | [66] |
AS: ATG AAA GTA CCC ATT TGG GG | ||||
KCC4 | S: AGG AAG CTG CTG AGC GCA C | AF087436 (mouse) | 443 | [66] |
AS: CAG CAT TGT ACA GGT GCA GC | ||||
WNK1 | S: ACC AGA AAG CCT CAT GTA AGC C | NM_053794 (rat) | 301 | This study |
AS: GTC CGC AGG GAA CGT CAT TG | ||||
WNK4 | S: CAC CTC CCG CCG CAA CAG | NM_175579 (rat) | 347 | This study |
| Â | AS: TCC ACA CAG CAA AGA GCA CCC | Â | Â | Â |