A-E Effects of starvation-refeeding on total 4E-BP1 · eIF4E complex (A), 4E-BP1 · phosphorylation (B) p70s6kinase phosphorylation (C) and mTOR phosphorylation in skeletal muscle tissue. Figure 3E is a representative blot, one of four, of each analysis. Proteins were immunoprecipitated with a monoclonal anti-eIF4E antibody and thereafter analyzed by Western blot with an antibody against 4E-BP1 in analysis of 4E-BP1 · eIF4E complex. Results are mean ± SEM with 7–9 animals per group. a-p<0.01 vs. freely fed and refed mice. All blot analyses were perfomed on the same protein extracts with the same amount of total protein applied among the groups (not shown).