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Table 2 Plasma amino acids in freely fed, starved and refed mice as described in material and methods

From: Liver-derived endocrine IGF-I is not critical for activation of skeletal muscle protein synthesis following oral feeding

   Freely fed Starved Refed Within groups p<0.05 Among groups p<0.05
   μmol/liter     
Essential AA       
  Isoleucine wt 61 ± 6 56 ± 4 67 ± 9 ns  
IGF- 59 ± 6 72 ± 7 50 ± 6 ns ns
  Leucine wt 124 ± 17 84 ± 6 179 ± 29 0.05  
IGF- 111 ± 12 114 ± 11 131 ± 20 ns ns
  Valine wt 161 ± 16 120 ± 6 176 ± 20 ns  
IGF- 152 ± 12 156 ± 13 143 ± 17 ns ns
  BCAA wt 346 ± 38 260 ± 16 422 ± 58 0.05  
IGF- 321 ± 28 342 ± 31 325 ± 43 ns ns
  Lysine wt 258 ± 16 246 ± 16 277 ± 14 ns  
IGF- 281 ± 21 282 ± 26 273 ± 21 ns ns
  Methionine wt 55 ± 7 41 ± 5 97 ± 10 0.001  
IGF- 48 ± 5 38 ± 3 77 ± 13 0.01 ns
  Phenylalanine wt 287 ± 37 389 ± 107 386 ± 93 ns  
IGF- 402 ± 86 277 ± 36 311 ± 72 ns ns
  Threonine wt 147 ± 14 119 ± 10 207 ± 13 0.05  
IGF- 125 ± 7 114 ± 8 162 ± 20 0.05 0.05
  Tryptophan wt 34 ± 4 30 ± 3 42 ± 4 ns  
IGF- 36 ± 3 35 ± 4 33 ± 3 ns ns
  Essential AA wt 1127 ± 80 1086 ± 119 1433 ± 89 0.05  
IGF- 1213 ± 112 1089 ± 70 1181 ± 91 ns ns
Non-essential AA       
  Alanine wt 337 ± 33 262 ± 37 547 ± 47 0.001  
IGF- 283 ± 26 194 ± 13 459 ± 44 0.001 0,01
  Arginine wt 77 ± 12 20 ± 7 43 ± 10 0.01  
IGF- 56 ± 14 41 ± 9 30 ± 10 ns ns
  Aspartic acid wt 37 ± 6 34 ± 3 25 ± 3 ns  
IGF- 22 ± 2 40 ± 6 33 ± 4 ns ns
  Asparagine wt 31 ± 2 27 ± 2 41 ± 2 0.01  
IGF- 25 ± 2 25 ± 1 32 ± 3 ns 0.01
  Citrulline wt 30 ± 3 20 ± 2 34 ± 2 0.01  
IGF- 29 ± 2 20 ± 1 30 ± 3 0.01 ns
  Glutamic acid wt 166 ± 18 152 ± 16 129 ± 15 ns  
IGF- 166 ± 11 138 ± 13 158 ± 23 ns ns
  Glutamine wt 538 ± 36 520 ± 30 578 ± 31 ns  
IGF- 553 ± 27 516 ± 42 560 ± 35 ns ns
  Glycine wt 174 ± 18 170 ± 17 178 ± 10 ns  
IGF- 150 ± 9 140 ± 15 175 ± 24 ns ns
  Histidine wt 53 ± 4 50 ± 3 70 ± 4 0.01  
IGF- 57 ± 4 52 ± 4 64 ± 4 ns ns
  Serine wt 108 ± 12 85 ± 5 136 ± 5 0.01  
IGF- 93 ± 16 75 ± 5 118 ± 9 0.01 ns
  Tyrosine wt 330 ± 23 395 ± 65 339 ± 82 ns  
IGF- 323 ± 39 239 ± 40 285 ± 59 ns ns
  Ornitine wt 88 ± 22 90 ± 12 109 ± 16 ns  
IGF- 109 ± 16 83 ± 15 115 ± 10 ns ns
  α-Aba wt 7 ± 1 8 ± 1 9 ± 1 ns  
IGF- 6 ± 1 6 ± 1 8 ± 1 ns ns
  Non-ess. AA wt 1977 ± 142 1835 ± 149 2237 ± 89 ns  
IGF- 1872 ± 71 1570 ± 112 2065 ± 125 0.05 ns
  Total AA wt 3103 ± 219 2921 ± 264 3669 ± 143 0.05  
   IGF- 3085 ± 171 2659 ± 180 3246 ± 205 0.08 ns
  1. Mean±SEM.
  2. wt = wild type mice.
  3. IGF- = hepatic deficient IGF-I (−/−) mice.