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Table 1 Summary of primers designed to amplify kcnq 1-5 and β-actin mRNAs

From: Kcnq 1-5 (Kv7.1-5) potassium channel expression in the adult zebrafish

Primer Nucleotide sequence Expected size (bp)
KCNQ1 fwd 5′- TCC AGT CGC TCA TGT GTC TC -3′ 203
KCNQ1 rev 5′- TTT CAT CCC ACC TTC TTT GC -3′  
KCNQ2 fwd 5′- GAG CCA GTG CAG GAG AAA AG -3′ 344
KCNQ2 rev 5′- TGA GGT AGA AGG CCG ACA CT -3′  
KCNQ3 fwd 5′- GAG AAG GAT TCG GCT CAC TG -3′ 443
KCNQ3 rev 5′- GCG TCT GCA TAG GTG TCA AA -3′  
KCNQ4 (a) fwd 5′-TAT GCA GAC TCC CTC TGG TG-3′ 175
KCNQ4 (a) rev 5′-CCT GCA CTT TCA GAG CAA AG-3′  
KCNQ4 (b) fwd 5′-GGG CCG CAG GGT TTC TTT AAA CTT-3′ 400
KCNQ4 (b) rev 5′-ATG ACA GTA TGC TGC CGT CCT TCA-3′  
KCNQ4 (c) fwd 5′-CGG CCG CAG GGT TTC TTT AAA CTT-3′ 400
KCNQ4 (c) rev 5′-TCC TTC AGT GGG AAG ATG GGC TTT-3′  
KCNQ4 ch19(a) fwd 5′-TGC CTG TAC AAT GTG CTG GAG AGA-3′ 265
KCNQ4 ch19(a) rev 5′-AAG GCT TTC TGG CAA AGC GTA GTC-3′  
KCNQ4 ch19(b) fwd 5′-ATC AGC CAA TGA TGA CAG ACG GGT-3′ 458
KCNQ4 ch19(b) rev 5′-AAG GCT TTC TGG CAA AGC GTA GTC-3′  
KCNQ5b fwd 5′- TGC CTG GTA TAT TGG GTT CC -3′ 261
KCNQ5b rev 5′- TGA ACC TTC AAG GCA AAA CC -3′  
β-actin fwd 5′- TCC CCT TGT TCA CAA TAA CC- 3′ 350
β-actin rev 5′- TCT GTG GCT TTG GGA TTC A-3′