Table 1 Genes and primer sequences used for qPCR analyses
From: Cardiac responses to elevated seawater temperature in Atlantic salmon
Gene | Dir. | Primer sequence (5′-3′) | GenBank acc. no. |
|---|---|---|---|
HSP701 | F | TGACGTGTCCATCCTGACCAT | BT043589.1 |
R | CTGAAGAGGTCGGAACACATCTC | ||
PGC1A2 | F | GTCAATATGGCAACGAGGCTTC | FJ710605 |
R | TCGAATGAAGGCAATCCGTC | ||
CPT13 | F | TCCCACATCATCCCCTTCAACT | AM230810 |
R | TGTCCCTGAAGTGAGCCAGCT | ||
HBB4 | F | ACAAACGTCAACATGGTCGACTGG | EG897325.1 |
R | TCTTTCCCCACAGGCCTACGAT | ||
HBA5 | F | AAGGCAGATGTCGTCGGTGCT | CK883845.1 |
R | CAGCCCAGTGGGAGAAGTAGGTCTT | ||
CD8A6 | F | CGTCTACAGCTGTGCATCAATCAA | AY693391 |
R | GGCTGTGGTCATTGGTGTAGTC | ||
SEC137 | F | AGTGGGCCTGTATCAGCGACGT | EG882700.1 |
R | ATCACTGCTCGTTCGTCGCTCC | ||
NDUFB88 | F | TCTGTCGCTGGGAGGAGAAGGA | DW532752.1 |
R | GTCCAGGCAGGTCCGATACTCTGT | ||
EF1A9 | F | CACCACCGGCCATCTGATCTACAA | AF321836 |
R | TCAGCAGCCTCCTTCTCGAACTTC |