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Figure 2 | BMC Physiology

Figure 2

From: Pharmacological and ischemic preconditioning of the human myocardium: mitoKATP channels are upstream and p38MAPK is downstream of PKC

Figure 2

Protocol for Study 2 to investigate the role of PKC, p38MAPK and mitoKATP channels in the cardioprotective effect of IP and PP with phenylephrine (P) or adenosine (A) (n = 6 specimens/group): (i) time-matched aerobic control, (ii) SI/R alone, (iii) IP alone prior to SI, (iv) chelerythrine (CHE) for 10 min prior to IP, (v) SB203580 (SB) for 10 min prior to IP, (vi) 5-hydroxydecanoate (5-HD) for 10 min prior to IP, (vii) phenylephrine (P) for 5 min and 5 min washout (W) before SI, (viii) chelerythrine (CHE) for 10 min prior to phenylephrine (P) for 5 min and 5 min washout (W), (ix) SB203580 (SB) for 10 min prior to phenylephrine (P) for 5 min and 5 min washout (W), (x) 5-hydroxydecanoate (5-HD) for 10 min prior to phenylephrine (P) for 5 min and 5 min washout (W), (xi) adenosine (A) for 5 min and 5 min washout (W) before SI, (xii) chelerythrine (CHE) for 10 min prior to adenosine (A) for 5 min and 5 min washout (W), (xiii) SB203580 (SB) for 10 min prior to adenosine (A) for 5 min and 5 min washout (W) and (xiv) 5-hydroxydecanoate (5-HD) for 10 min prior to adenosine (A) for 5 min and 5 min washout (W).

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