Figure 3
Study 3 protocol designed to elucidate the sequence of the participation of involvement of mitoKATP channels, PKC and p38MAPK in the signal transduction cascade of cardioprotection. For this purpose, in addition to the (i) aerobic time-matched control and (ii) SI/R alone the following groups were studied (n = 6 specimens/group): (iii) diazoxide alone for 10 min prior to SI, (iv) chelerythrine (CHE) for 20 min with diazoxide added for the last 10 min prior to SI, (v) SB203580 (SB) for 20 min with diazoxide added for the last 10 min prior to SI, (vi) PMA alone for 10 min prior to SI, (vii) SB203580 (SB) for 20 min with PMA added for the last 10 min before SI, (viii) 5-hydroxydecanoate (5-HD) for 20 min with PMA added in the last 10 min before SI, (ix) anisomycin (ANS) alone for 10 min prior to SI, (x) chelerythrine (CHE) for 20 min with anisomycin (ANS) added for the last 10 min prior to SI and (xi) 5-hydroxydecanoate (5-HD) for 20 min with anisomycin (ANS) added for the last 10 min prior to SI.