Figure 1

Changes in total DAG mass in liver and liver cell plasma membranes following treatment of rats with mercazolil or thyroxine. A, liver homogenates. B, isolated liver cell plasma membranes. T₄ (200 μg/100 g weight) was injected to the normal rats three times a week. The last hormone injection was made 48 h prior to killing. Control rats received the same volume of 0.9% NaCl. Mercazolil was injected to the animals and lipids were extracted and separated as described in "Materials and Methods". The intensity of lipid spots was estimated by densitometry using a LKB Ultroscan laser densitometer. Results are mean ± S.E. of four individual experiments. ^* P < 0.05 vs. 90-day-old rats, ^** P < 0.001 vs. control rats, ^*** P < 0.001 vs. mercazolil-treated rats.