Figure 3
[14C]-DAG accumulation in liver slices of rats of different age and thyroid status. Mercazolil was injected to the animals as described in "Materials and Methods". T4 (10 μg/100 g weight) was injected to the mercazolil-treated rats 48 h prior to killing. Control rats received the same volume of 0.9% NaCl. The liver slices were labeled by incubation in Eagle medium containing 10% fetal calf serum, 100 units/liter streptomycin, 100 units/liter penicillin, 13 mg/ml gentamycin and 2.5 μCi/ml of [14C]oleic acid for 1 h in 95% O2/5% CO2 atmosphere at 37°C. The lipids were extracted and analyzed as described in "Materials and Methods". Results are mean ± S.E. of six individual experiments. * P < 0.05 vs. 90-day-old rats, ** P < 0.05 vs. control rats, *** P < 0.05 vs. mercazolil-treated rats.