Figure 3

U73343 rapidly blocks and partially reverse the effects of MTX. Five traces are shown superimposed in each panel. Upper panel. Fura-2 loaded BAECs were stimulated with MTX at 100 sec. U73343 (5 μM) was subsequently added at either 200 (red), 300 (green), 400 (yellow), or 500 sec (blue). Lower panel. EB uptake in BAECs with the same protocol and trace colors as the upper panel are shown. Results shown are representative of 3 independent experiments.