Regional differences in expression of VEGF mRNA in rat gastrocnemius following 1 hr exercise or electrical stimulation

Table 1 Normalized¹ mean VEGF₁₆₅ signal from in situ hybridization in fibers randomly sampled from oxidative and glycolytic regions of the rat gastrocnemius. Values are given as mean ± SE.

	Oxidative region		Glycolytic region
	Number of fibers sampled	VEGF signal (Mean of fibers sampled)	Number of fibers sampled	VEGF signal (Mean of fibers sampled)
Treadmill
Muscle 1	27	1.00 ± 0.05	27	0.93 ± 0.05
Muscle 2	27	1.00 ± 0.03	27	0.95 ± 0.02
Muscle 3	27	1.00 ± 0.03	27	0.92 ± 0.03
Muscle 4	27	1.00 ± 0.02	27	0.91 ± 0.01
Mean²		1.00 ± 0.00		0.93 ± 0.01^*
Electrical
Muscle 1	27	1.00 ± 0.04	27	0.76 ± 0.03
Muscle 2	27	1.00 ± 0.11	27	0.63 ± 0.14
Muscle 3	27	1.00 ± 0.09	27	0.65 ± 0.06
Muscle 4	27	1.00 ± 0.69	27	0.31 ± 0.25
Mean²		1.00 ± 0.00		0.59 ± 0.1^*
Control
Muscle 1	27	1.00 ± 0.04	27	0.83 ± 0.04
Muscle 2	27	1.00 ± 0.03	27	0.99 ± 0.05
Mean²		1.00 ± 0.00		0.93 ± 0.02

¹Normalized within each muscle sample to the mean of the oxidative region. ²ANOVA mean using individual muscles as the unit of analysis (P = 0.002 for regional differences and P = 0.036 for protocol differences). ^*Significantly different from mean of oxidative region by paired t-test, p ≤ 0.05.

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