Figure 1

Osmotically induced swelling in oocytes expressing AQP1 wild type and C-terminal mutant channels. A. Relative volume increases were determined by videomicroscopy as a function of time after the transfer of the oocytes from 200 mOsM into 100 mOsM saline at time 0. The change in cross-sectional area of the oocyte was converted to volume to calculate relative volume increase and standardized to the initial volume at time zero. Data are shown for representative individual oocytes. Panels show data for control and AQP1 wild type- or mutant-expressing oocytes from within the same batches of oocytes. B. Summary of the osmotic water permeability (Pf) values for wild type and mutant channels. Data for Pf (cm/s × 10^-4) are mean ± SEM; n values are shown in parentheses (right). Unpaired two-tailed Student's t tests were used to analyze significance. An asterisk (*) indicates a value that is significantly different (p < 0.0005) from control; the pound sign (#) indicates a value that is significantly different (p < 0.009) from wild type AQP1.