Figure 5

Summary of the effects of AQP1 C-terminal mutations on the net conductance responses evoked by SNP. Data are grouped to compare control, AQP1 wild type and mutant responses from within the same batches of oocytes. The mutants D237S, D237N (A) and D237K (B) showed dose-dependent responses to SNP (mM) comparable to that of wild type AQP1. However, the mutations D237A (A) and K243C (C) showed decreased conductance responses at all doses of SNP as compared with wild type. 'Initial' data are the conductance levels of the oocytes recorded prior to SNP application, and represent total conductance rather than net values. The net conductance responses were calculated by subtracting the matched initial values individually from the SNP response for each oocyte. Data are mean ± SEM; n = 3–28 oocytes per group. An asterisk (*) indicates values that are significantly different (p < 0.02) from control; the pound sign (#) indicates values that are significantly different (p < 0.04) from wild type AQP1 (unpaired two-tailed Student's t test).