Figure 6

Double mutation D237A + K243C in the AQP1 carboxyl terminal domain shows a decreased conductance response to SNP as compared with wild type. A. Net current responses of control oocytes, and oocytes expressing AQP1 wild type and mutant channels were measured for voltage steps from +60 mV to -110 mV in -10 mV intervals, from -40 mV holding potential, after 4 minutes in 8 mM SNP. B. Summary of conductances (mean ± SEM, n = 9–18 oocytes per group) measured at different doses of SNP (mM). 'Initial' data are the conductance levels of the oocytes recorded prior to SNP application. The net conductance responses were calculated by subtracting the matched initial values individually from the SNP response for each oocyte. An asterisk (*) indicates values that are significantly different (p < 0.006) from control; the pound sign (#) indicates values that are significantly different (p < 0.0001) from wild type AQP1 (unpaired two-tailed Student's t test).