Table 1 List of primers used for the site-directed mutations of selected amino acids in the carboxyl terminal domain of Aquaporin-1.
Mutation | Primer | Position | Sequence (mutation underlined) |
|---|---|---|---|
D237S | Sense | bp 726-759 | 5'-GGCCCCACGCAGCAGTAGC CTCACAGACCGCGTG-3' |
D237S | Antisense | bp 759-726 | 5'-CACGCGGTCTGTGAGGCT ACTGCTGCGTGGGGCC-3' |
D237N | Sense | bp 725-759 | 5'-TGGCCCCACGCAGCAGTAAC CTCACAGACCGCGTG-3' |
D237N | Antisense | bp 759-725 | 5'-CACGCGGTCTGTGAGGTT ACTGCTGCGTGGGGCCA-3' |
D237K | Sense | bp 727-759 | 5'-GCCCCACGCAGCAGTAAG CTCACAGACCGCGTG-3' |
D237K | Antisense | bp 759-727 | 5'-CACGCGGTCTGTGAGCTT ACTGCTGCGTGGGGC-3' |
D237A | Sense | bp 726-760 | 5'-GGCCCCACGCAGCAGTGCC CTCACAGACCGCGTGA-3' |
D237A | Antisense | bp 760-726 | 5'-TCACGCGGTCTGTGAGGGC ACTGCTGCGTGGGGCC-3' |
K243C | Sense | bp 744-778 | 5'-CCTCACAGACCGCGTGTGC GTGTGGACCAGCGGCC-3' |
K243C | Antisense | bp 778-744 | 5'-GGCCGCTGGTCCACACGCA CACGCGGTCTGTGAGG-3' |
D237A + K243C | Sense | bp 744-778 | 5'-CCTCACAGACCGCGTGTGC GTGTGGACCAGCGGCC-3' |
D237A + K243C | Antisense | bp 778-744 | 5'-GGCCGCTGGTCCACACGCA CACGCGGTCTGTGAGG-3' |