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Figure 4 | BMC Physiology

Figure 4

From: Heat induced HSP20 phosphorylation without increased cyclic nucleotide levels in swine carotid media

Figure 4

Cyclic nucleotide levels did not correlate with heat induced ser16-HSP20 phosphorylation levels. Swine carotid artery tissues were either 1) stimulated with 10 μM histamine for 10 min at 37°C or 2) 44.5°C for 4 hours and then stimulated with 10 μM histamine for 10 min at 44.5°C prior to freezing. Histamine induced [cGMP] is shown in the top panel, [cAMP] in the center panel, ser16-HSP20 phosphorylation (mol Pi at ser 16 / mol HSP20) in the third panel, and force in the bottom panel (n = 4, data presented as mean ± 1 SEM). The ser16-HSP20 phosphorylation data is from a second set of tissues treated identically (the ''before'' group from Fig. 2). Treatment at 44.5°C did not significantly alter [cAMP] and [cGMP] levels despite significantly increasing ser16-HSP20 phosphorylation and suppressing histamine induced force.

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