Figure 6

Dual labeling of E-cadherin and aquaporin 1 or aquaporin 2 in the outer cortex. Samples were processed for the dual labeling of E-cadherin and either aquaporin 1 or aquaporin 2 as described in the Methods and viewed using either a 40× or a 100× objective. Panels A and B show dual labeling of aquaporin 1 (A) and E-cadherin (B) in the same field. Panels C and D show the dual labeling of aquaporin 2 (C) and E-cadherin (D). CD denotes collecting ducts and DT denotes distal tubules. (E) E-cadherin labeling in a collecting duct. (F) E-cadherin labeling in a distal tubule. Original magnification = 174× (Panels A-D) and 435× (Panels E and F).