Figure 3
The effects of bath pH and intracellular [cAMP] on sAC expression. A: RT-competitive PCR of sAC mRNA from cultured BCECs exposed to different extracellular pH. From left to right: M: Marker; pH: 7.0, 7.5 and 8.0. B. immunoblots of sAC protein expression and β-actin from Calu-3 cells exposed to HCO3--free culture medium at pH 7.0, 7.5 or 8.0. C: RT-competitive PCR of sAC mRNA from cultured BCECs exposed to 10 μM adenosine or 10 μM forskolin in the absence of bicarbonate. From left to right: M: Marker; SBF: standard bicarbonate free media; Ado: adenosine; FSK: forskolin. D: summary of the ratios of band densities (sAC/GAPDH) from A and C. Error bar indicates ± SE (n = 3).