Figure 4From: [HCO3-]-regulated expression and activity of soluble adenylyl cyclase in corneal endothelial and Calu-3 cellsThe effects of bath osmolarity and additional [NaCl] on sAC expression. RT-competitive PCR was performed using specific sAC primers and GAPDH internal standard primers from cultured BCECs. A: RT-competitive PCR of sAC mRNA from cultured BCECs exposed to different osmolarity (sucrose added). From left to right: M: Marker; osmolarity: 263, 272, 290, 321, 359, 383 (mmol/kg). B: summary of the ratios of band densities (sAC/GAPDH) from A. Error bar indicates ± SE (n = 3). C: RT-competitive PCR of sAC mRNA from cultured BCECs exposed to different [NaCl]. From left to right: M: Marker; [NaCl]: 10, 20, 40, 60, 80 (mM). D: summary of the ratios of band densities (sAC/GAPDH) from C. Error bar indicates ± SE (n = 3).Back to article page