Figure 5

The effects of HCO ₃ ^--regulated sAC expression on intracellular cAMP accumulation. Culture medium was removed from confluent cultured BCECs and replaced with HCO₃^--free DMEM for 48 hours at 37°C. Cells were then incubated either for different time in 40 mM HCO₃^- DMEM or for 24 hours in different [HCO₃^-] at 37°C. Cells were lysed in 0.1 N HCl. [cAMP] was measured by enzyme immunoassay. A: changes in intracellular [cAMP] from cells exposed to 40 mM HCO₃^- with 0, 3, 6, 12, 24 and 48 hour incubation. B: Intracellular [cAMP] from cells exposed to 0, 5, 10, 20, 40, 60 and 80 mM HCO₃^- for 24 hour incubation. Error bars indicate ± SE (n = 6)