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Figure 6 | BMC Physiology

Figure 6

From: [HCO3-]-regulated expression and activity of soluble adenylyl cyclase in corneal endothelial and Calu-3 cells

Figure 6

The effect of HCO 3 --induced sAC expression on apical Cl- permeability in BCECs A: HCO3--starved BCECs grown on permeable Anodisc. Both apical (AP) and basolateral (BL) compartments were initially perfused with Cl-- and HCO3--free Ringer solution. After the 1st apical (AP) Cl- pulse, HCO3--rich Ringer solution (BR) was introduced on both sides for at least 5 min before the 2nd Cl- pulse. Break in trace indicates period of wash in Cl--free and BR solution until trace stabilized (at least 5 min). B: maximum slope summary data for A (n = 5); all fluorescence values were normalized to the fluorescence value in the absence of Cl- (F0) obtained just before addition of Cl-. Calculated slopes were adjusted by any background drift in the fluorescence trace that was apparent just before addition of Cl-. C: BCECs cultured in BR medium, same experiment as in A. D: summary data for C (n = 5). *Significantly different from HCO3--free solution (BF) (P < 0.05).

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