Figure 1

Characterization of the antibodies used in this study. Rat heart membrane fractions were separated on 8–12% denaturing PAGE and Western blotting was performed using Kir6.1 (NAF1, CAF-1 or C-16) or Kir6.2 (G-16 or 76A) antibodies. In other experiments, lysates of HEK293 cells stably transfected with Kir6.1/SUR2B, or lysates of COS7L cells transiently transfected with SUR1, SUR2A or SUR2B cDNAs, were separated with PAGE and respectively immunoblotted with anti-SUR2 (C-15) or anti-SUR1 (C-16) antibodies. Molecular size markers are indicted as appropriate.