Figure 2

Immunohistochemistry demonstrating the regional distribution of Kir6.1 subunits in cryostat sections of the mouse ventricle. A: The low magnification image demonstrates regional expression differences with more prominent myocyte staining apparent in the epicardium (left) than in the mid-myocardium (right). Image width is 750 μm. B and C: Higher magnification of the same slide shown in previous panel showing an epicardial (B) and midmyocardial section (C). The image widths are respectively 102 and 96 μm. D: Double stained section of a midmyocardial section using NAF-1 anti-Kir6.1 antibodies (D1) and ICAM-2 antibodies as a marker of endothelial cells. (D2). The image width is 119 μm. The secondary antibodies used were Cy-3 conjugated donkey anti-rabbit and Cy-5 conjugated donkey anti-rat F(ab')2 IgG fragments.