Figure 6

Effect of different fixation protocols on immunocytochemistry of ventricular myocytes. Enzymatically isolated ventricular myocytes were subjected to fixation protocols with (A) methanol, (B) paraformaldehyde or (C) sequential double fixation with paraformaldehyde followed by methanol. The primary antibody in all cases was against Kir6.2 subunits (76A) and the secondary antibody was Cy-3 conjugated donkey anti-rabbit IgG. The image widths are respectively 137, 107 and 139 μm.