Figure 8
Subcellular localization of Kir6.1, Kir6.2 and SUR1 subunits in ventricular myocytes. A: Co-staining of a mouse ventricular myocyte with antibodies to Kir6.1 (A1: NAF-1) or SUR1 (A2: C-16). The secondary antibodies were Cy2-conjugated donkey anti-rabbit and Cy3-conjugated donkey anti-goat. The degree of co-localization after background elimination is depicted in panel A3 by plotting pixel intensities of the two channels against each other. Pseudo-coloring of pixels indicates degree of co-localization (black is low and white high). B: Co-staining of a mouse cardiac myocyte with anti-Kir6.2 antibodies (76A) and anti SUR1 antibodies. Secondary antibodies used were Cy-2 conjugated donkey anti-rabbit to detect Kir6.2 (green) and Cy-3 conjugated donkey anti-goat IgG to detect SUR1 (red). A 3-D reconstruction was performed (58 stacked images with a voxel size of 0.23 μm3). The image width is 119 μm. The images shown on the bottom and right insets are cut-through projections of the cell width (the regions of cross-sections areas shown by the white lines; the middle of each image is also indicated by a white line for clarity).