Figure 9
Subcellular localization of KATP channel subunits in isolated rat ventricular myocytes. A: Staining with NAF-1 anti-Kir6.1 antibodies (detection with Cy-3 conjugated donkey anti rabbit). Image width is 128 μm. B: Staining with a different anti-Kir6.1 antibody (C16, Santa Cruz); secondary antibody used was Cy-3 conjugated donkey anti-goat IgG. Image width is 136 μm. C: Staining with an anti-SUR1 antibody. Image width is 128 μm. D: Co-localization of Kir6.2 and SUR2 subunits. The preparation was co-stained with an anti-Kir6.2 antibody (76A; detection with Cy-2 conjugated donkey anti-rabbit IgG; green) and SUR2 antibodies (detected with Cy-5 conjugated donkey anti-goat IgG; pseudo-colored red for clarity). Panel D3 is an overlay of panels D1 and D2; yellow is indicative of co-localization. Image width is 125 μm.