Figure 1

Lower CK isoform expression and activity in rat extraocular muscle. (A) Relative abundance of three CK isoform mRNAs in EDL and extraocular muscle by quantitative PCR. Each sample includes pooled muscles from 4 rats. Data are means from 3–4 samples, and are normalized to levels measured in EDL = 100%. Message levels for the cytosolic CK-M and CK-B, and the mitochondrial sCK were much lower in extraocular muscles compared to EDL. (B) Total CK activity in EDL and extraocular muscles. EDL muscles have ~5-fold greater CK activity than extraocular muscles (n = 8, P < 0.001). (C) CK protein isoforms found in brain, EDL and extraocular muscle by western blot.