Figure 2

Myomesin and M-protein are downregulated in rat extraocular muscles. (A) Representative confocal micrographs showing that myomesin and M-protein are found in EDL muscle fibers (EDL, top) in the same stereotypical banding pattern, but are not detectable in extraocular muscle (EOM, bottom). Scale bar = 20 μm. (B) Western blots demonstrating the presence of myomesin and M-protein in EDL and heart (H). Embryonic heart (He) did not have M-protein, but two bands reacted with the myomesin antibody: myomesin and EH-myomesin, an alternatively spliced isoform of higher molecular weight. Extraocular muscle (EOM) contained EH-myomesin, but did not have detectable levels of myomesin or M-protein.