Figure 2

Immunoblots for aquaporins and canalicular solute transporters in WIF-B cells. A, 50 μg of plasma (PM) and intracellular (IM) membranes from WIF-B cells were loaded onto 12% SDS-polyacrylamide gels. Immunoblots were performed using affinity-purified rabbit anti-AQP primary antibodies and goat anti-rabbit secondary antibodies and visualized via chemiluminescence. B, 50 μg of PM and IM from WIF-B cells were loaded onto 8% SDS-pliacrylamide gels. Immunoblots were performed using affinity-purified goat anti-Mrp2 or Bsep and rabbit anti-AE2. Rat hepatocyte homogenate (20 μg) were used as positive controls.