Figure 1

The steady-state relationship between active force and percent MRLC phosphorylation in the rabbit urinary bladder detrusor strips. Force is expressed as a percent of the initial response to 3 μM carbachol. The open circles show results from tissues were stimulated by varying doses of carbachol (0.01, 0.03, 0.1, 0.3, 1.0, 3.0, 10, 30, and 100 μM) until steady state was achieved (the data with carbachol alone previously published [11]). The symbol at 56% of force represents tissues stimulated with 0.3 μM carbachol. The filled symbols show results from tissues were stimulated 0.3 μM carbachol until steady state was achieved followed by addition of 300 μM 8-bromo-cGMP (filled circle), 10 μM forskolin (filled square), or 0.3 μM isoprenaline (filled triangle) until steady state was achieved.