Figure 2From: Effect of bradykinin on nitric oxide production, urea synthesis and viability of rat hepatocyte culturesDetermination of urea production after treatment of hepatocytes with bradykinin. Urea production was spectrophotometrically determined at 600 nm after 2 h incubation with 1 mM NH4Cl in basal conditions (white column), in presence of 0.01 mM bradykinin (hatched column), in presence of 0.1 mM bradykinin (crosshatched column) and in presence of 0.1 mM bradykinin with 1.68 mM L-NAME (black column). Values, expressed as ng urea per cell per hour, are the means ± S.E.M. (bars) of four independent experiments.p < 0,05 compared with control Back to article page