Figure 3

Determination of cell viability in hepatocytes treated with bradykinin. The cell viability was spectrophotometrically determined at 570 nm by MTT assay in hepatocytes incubated in basal conditions (white column), in presence of 0.01 mM bradykinin (hatched column), in presence of 0.1 mM bradykinin (crosshatched column) and in presence of 0.1 mM bradykinin with 1.68 mM L-NAME (black column) for 2 h period. (A) Cell viability determined immediately after. (B) Cell viability determined after an additional 24 h incubation period in incomplete medium Results are expressed as a percentage of control. Values are the means ± S.E.M. (bars) of four independent experiments. p < 0,05 compared with control. p < 0,05 compared with control and 0.1 mM bradykinin treated cells