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Table 1 Effect of bradykinin treatment on cell viability

From: Effect of bradykinin on nitric oxide production, urea synthesis and viability of rat hepatocyte cultures

 

Treatment: 2 h+Brad (Viability %)

Treatment: 2 h+Brad + 24 h-Brad (Viability %)

Control

100 ± 10

100 ± 6

0.01 mM Bradykinin

98 ± 12

104 ± 10

0.1 mM Bradykinin

104 ± 8

50 ± 11*

0.1 mM Bradykinin + 1.68 mM L-NAME

102 ± 10

79 ± 7**

  1. Hepatocytes were isolated and cultured in presence and in absence of bradykinin (either 0.01 mM or 0.1 mM) and cotreated with bradykinin 0.1 mM and L-NAME 1.68 mM for a 2 h period. Cell viability was determined by Trypan blue exclusion test either immediately after 2 h or after additional 24 h incubation period in incomplete medium. Results are expressed as a percentage of control. Values are expressed as mean ± S.E.M., n = 4.
  2. *Statistically significant differences (p < 0,05) from control levels as determined by Student's t-test.
  3. ** Statistically significant differences (p < 0,05) from control levels and from 0.1 mM bradykinin treated cells as determined by Student's t-test.