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Figure 5 | BMC Physiology

Figure 5

From: PKC translocation and ERK1/2 activation in compensated right ventricular hypertrophy secondary to chronic emphysema.

Figure 5

A. Protein kinase C (PKC) immunoreactivity during right ventricular hypertrophy in hamsters with emphysema. Membranous (Mem) and cytosolic (Cyt) fractions were prepared from hamster right ventricle. Representative immunoblots for PKC ε and PKC βI demonstrate increased translocation in Emp, while PKC α and βII were unchanged. Translocation was quantified by taking ratio of membrane to cytosolic levels (M:C) of each isoform. Average PKC ε translocation index (of three separate trials) was 1.14 ± 0.11 for Con (n = 5) and 2.22 ± 0.33 for Emp (n = 5) animals. B. Graph of M:C illustrates fold change normalized to controls. Fold changes are averages of three separate experiments for each PKC isoform. C. Levels of total phosphorylated PKC ε from whole right ventricular homogenates were unchanged among Con and Emp hamsters. *p ≤ 0.05. Con, control; Emp, emphysema.

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