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Table 2 The primers, amplicon sizes and annealing temperatures used for gene expression measurements by real-time PCR.

From: Exploring hepatic hormone actions using a compilation of gene expression profiles

gene

left primer

right primer

size

temp

FAT/CD36

GCAACAACAAGGCCAGGTAT

TGTGGCTGAGCAGAAAGAGA

200

54

Ppp3ca

GCAGGCTGGAAGAAAGTGTC

AAGGCCCACAAATACAGCAC

200

54

Hsd11b1

TTTTGCAGAGCGATTTGTTG

TGCTCAGGACCACATAGCTG

200

54

Phyh

TACGTGGAGTGCTTCACTGG

CCATTGTTCCTGTCGATGTG

200

54

Srebp1a

GCGCCATGGACGAGCTG

TTGGCACCTGGGCTGCT

200

57

Srebp1c

GGAGCCATGGATTGCACATT

GCTTCCAGAGAGGAGGCCAG

200

54

Srebp2

CCCTTGACTTCCTTGCTGCA

GCGTGAGTGTGGGCGAATC

200

54

  1. The expression of phytanoyl-CoA hydroxylase (Phyh), hydroxysteroid 11-beta dehydrogenase 1 (Hsd11b1), the catalytic subunit of protein phosphatase 3, alpha isoform (Ppp3ca), fatty acid translocase/cd36 antigen (FAT/CD36) and sterol regulatory element-binding proteins (SREBPs) 1a, 1c and 2 were measured using the primers shown in the Table. To allow comparison between samples, the expression data for each of the genes was normalized to the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which was always run in parallel.