Genes | in vivo | hepatocytes |
---|
 | Array | RT-PCR | Array | RT-PCR |
---|
Phyh | 0.34 | 0.6 | 0.54 | 0.6 |
Hsd11β1 | 0.31 | 0.28 | 0.63 | 0.5 |
Ppp3ca | 1.33 | 1.43 | 1.48 | 1.33 |
CD36 | 2.39 | 2.78 | 1.26 | 2.07 |
- Microarray and RT-PCR expression measurements for phytanoyl-CoA hydroxylase (Phyh), hydroxysteroid 11-beta dehydrogenase 1 (Hsd11β1), the catalytic subunit of protein phosphatase 3, alpha isoform (Ppp3cα) and fatty acid translocase/CD36 antigen (FAT/CD36). The real-time PCR results were normalized to GAPDH. The ratios between treated and untreated samples in vivo (young male rats treated with bGH or vehicle for 1 week via minipumps, n = 4 per group), and the ratios between GH-treated (24 hours) and untreated primary hepatocytes are shown.