Figure 6

The PI3K inhibitors wortmannin and LY-294002 inhibit the EFS-induced slow membrane depolarization in canine isolated mesenteric vein. Intracellular recordings of membrane potential in response to EFS (0.1 ms, 13 V, 10 s) in canine isolated mesenteric veins preincubated for 60 min prior to and throughout the EFS with 100 nM wortmannin (A) or 10 μM LY-294002 (B). The experimental data are presented as mean ± SEM, n = 3–4 (C). *, P < 0.05 vs control. Scale bars apply to traces A and B.