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Figure 1 | BMC Physiology

Figure 1

From: Proinflammatory cytokines tumor necrosis factor-α and interferon-γ modulate epithelial barrier function in Madin-Darby canine kidney cells through mitogen activated protein kinase signaling

Figure 1

Effect of Inflammatory Cytokine Concentration on MDCK Cell Barrier Function. Lactate dehydrogenase release (Panel A) was measured in confluent MDCK cell cultures 24 hours following exposure to increasing doses of TNFα and IFNγ. Results are expressed as percent of maximal LDH release determined by incubating MDCK cells with TX-100 (2%) five minutes prior to LDH activity assay. The mean LDH is reported, error bars represent the SE, four independent experiments were assayed in duplicate. Fluorescein flux (Panel B) was measured following 24 hour treatment with increasing dose of TNFα and IFNγ. Fluorescein (50 μM) was added to the apical chamber and recovery was measured from the basal chamber after a 120 minute incubation. The mean fluorescence is reported, error bars represent the SE of four independent experiments. A one-way analysis of variance (ANOVA) was performed, multiple comparisons between control and treatments were determined with the Bonferroni post test. **Indicates statistical difference (P < 0.001) to control.

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BMC Physiology

ISSN: 1472-6793

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