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Figure 5 | BMC Physiology

Figure 5

From: Proinflammatory cytokines tumor necrosis factor-α and interferon-γ modulate epithelial barrier function in Madin-Darby canine kidney cells through mitogen activated protein kinase signaling

Figure 5

Effect of Proinflammatory Cytokine Concentration on MDCK Cell Tight Junction Proteins. Representative immunoblots of occludin, claudin-1, claudin-2 and claudin-3 total protein from confluent MDCK cell cultures twenty-four hours following exposure to increasing doses of TNFα and IFNγ (Panel A). Immunoblots were subjected to densitometric analysis; results are reported as percent of control to examine the effect of proinflammatory cytokines on tight junction protein expression (Panel B). Dashed horizontal lines represent control level of expression (100%), error bars represent the mean ± SE of eight independent experiments. ANOVA was performed, multiple comparisons between control and treatments were determined with the Bonferroni post test. **Indicates statistically difference (P < 0.01) to control.

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BMC Physiology

ISSN: 1472-6793

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