Skip to main content
Figure 6 | BMC Physiology

Figure 6

From: Proinflammatory cytokines tumor necrosis factor-α and interferon-γ modulate epithelial barrier function in Madin-Darby canine kidney cells through mitogen activated protein kinase signaling

Figure 6

ERK1/2 Inhibition Reverses Cytokine-induced Alterations in Tight Junction Protein Distribution. Representative immunoblots of occludin and claudin-1 using Triton X-100 soluble and insoluble (SDS) fractions from confluent MDCK cell cultures treated for 24 hours in the indicated conditions (Panel A). The effect of the MEK inhibitor (U0126, 1 μM) was added fifteen minute prior to addition of proinflammatory cytokines. Densitometic analyses were performed, occludin (filled bars) and claudin-1 (open bars), ratio of SDS to TX-100 intensity was reported. Error bars represent the mean ± SE of four independent experiments. ANOVA was performed, multiple comparisons between all treatments were determined with the Tukey-HSD post test. **Indicates statistically difference (P < 0.05) to the control group, ***indicates a significant difference to the TNFα/IFNγ group (P < 0.05).

Back to article page

BMC Physiology

ISSN: 1472-6793

Contact us