Table 1 Determination of Apoptosis in MDCK Monolayers. Confluent MDCK cultures were placed in one of the indicated treatment groups for 24 hours. Cells were then fixed and apoptotic cells were label using the TUNEL technique. Fluorescent microscopy was used to analysze of total cell number by DAPI staining and apoptotic cells by fluorescein incorporation. The mean ± SE of TUNEL positive cells is reported from three independent experiments. ANOVA was performed followed by a Bonferroni post test with all comparisons against the cells in media containing 5% FBS and glucose, *P < 0.01.

From: Proinflammatory cytokines tumor necrosis factor-α and interferon-γ modulate epithelial barrier function in Madin-Darby canine kidney cells through mitogen activated protein kinase signaling