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Figure 4 | BMC Physiology

Figure 4

From: Streptozotocin-induced diabetes in the rat is associated with changes in vaginal hemodynamics, morphology and biochemical markers

Figure 4

Effect of diabetes on arginase I, eNOS, and PKG protein expression in the rat vagina. Vaginal tissues from diabetic (Diab) or age-matched control (Con) rats were homogenized and the cytosolic fraction was obtained by differential centrifugation. The vaginal tissue cytosolic extract (200μg total protein/lane) was then subjected to gel electrophoresis under denaturing conditions. Gels were blotted onto nitrocellulose membranes that were probed with primary antibodies against arginase I, eNOS and PKG. After incubation with the secondary antibody, membranes were developed using an enhanced chemiluminescence reaction and bands were visualized by exposure to film.

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